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DNA Profiling - an overview | ScienceDirect Topics Thus, the DNA profile of the majority of seafood species need to be analysed and curated Nevertheless, the method is able to show that a seafood product has been mislabelled or substituted Similarly, Carrera et al (2000) used PCR to identify and compare the DNA profile of Atlantic salmon (S salar) and rainbow trout (O mykiss)
DNA Profiling - an overview | ScienceDirect Topics The development of what have been characterized as “sample-to-profile” and microfluidic Lab-on-a-Chip technologies offer an opportunity to finally achieve rapid DNA profiling in the field by fully integrating DNA extraction, purification, template input, STR amplification, amplicon size fractionation, allele detection, and genotype analysis (Lounsbury et al , 2013; Greenspoon et al , 2008
The interpretation of single source and mixed DNA profiles Binary methods of DNA interpretations, which rely on a rule and threshold based system have been pushed to their limits, and it seems that the next step for DNA profile interpretation is to move to continuous systems Continuous systems can utilise all the currently observed information within a DNA profile
How many cells are required for successful DNA profiling? Due to inefficient collection of the DNA template present by swabbing or tapelifting techniques and the loss of DNA through standard extraction methods, reported to range between 20 %–80 % [5, 6], far more than 80 diploid cells would need to be collected to ensure a full profile downstream given the optimum template stated [[1], [2], [3]]
Plasma DNA Profile Associated with DNASE1L3 Gene . . . - ScienceDirect For example, the size profile of plasma DNA typically shows a 166 bp major peak with 10 bp periodicities occurring from approximately 143 bp and below 6, 9 Circulating fetal and tumor DNA molecules have been found to have a shorter size distribution than that of the background DNA 5, 6, 9 Additionally, certain sequence motifs are more frequently observed at the ends of plasma DNA molecules
DNA profile match probability calculation: how to allow for population . . . ELSEVIER SCIENTIFIC PUBLISHERS IRELAND Forensic Science International 64 (1994) 125-140 DNA profile match probability calculation: how to allow for population stratification, relatedness, database selection and single bands David J Balding*, Richard A Nichols School of Mathematical Sciences, School of Biological Sciences, Queen Mary and Westfield College, University of London, Mile End Road
Composite profiles in DNA analysis - ScienceDirect Composite profiles are created by combining DNA profiling information from replicate profiles derived from the same DNA extract In this paper we have shown that, provided the probability of drop-in is low or nil, the creation of composite profiles is an acceptable approximation to a Bayesian approach, as long as simple samples are analysed
Evaluating forensic DNA profiles using peak heights, allowing for . . . Caseworkers in criminal investigations are often faced with the task of evaluating a crime-scene DNA profile in terms of hypotheses about the identity of at least one unknown source of the DNA, using reference profiles from known individual(s) relevant to the case and anonymous databases for estimation of population frequencies
Investigating a common approach to DNA profile interpretation using . . . This DNA profile, whilst having all alleles represented within the mixed DNA profile, could be readily excluded as a plausible contributor given an assumption of two contributors At this point two approaches to interpretation were observed: either to progress an LR calculation ( LR = 0) to underpin a conclusion of exclusion, or to make an exclusionary statement without a supporting statistic
DNA - an overview | ScienceDirect Topics All DNA fingerprinting techniques produce a pattern or profile of nucleic acids amplified (by PCR) from a sample and that pattern reflects the microbial community structure from an environment (Kitts, 2001) DNA fingerprinting or profiling comprises any DNA-based techniques that identifies the DNA from a certain individual or group of individuals within a community of organisms