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Doyle, J. J. and Doyle, J. L. (1987) A Rapid DNA Isolation . . . The protocol developed yielded 200 - 1000 ng μl of quality DNA without any impurities as evident by A260 280 ratio ranging from 1 75 - 2 0 It was also suitable for extracting quality DNA from other members of Meliaceae like Azadirachta indica and Melia azedarach
Charlotte We here describe a simple modification of this procedure that has been found to be efficient for nucleic acid isolations from fresh leaf tissue, and which We have also used for dried herbarium specimens
Something old, something new, a DNA preparation procedure for . . . Here we describe a DNA preparation protocol that our research group routinely uses to obtain high yields of HMW DNA from the freshwater microcrustacean Daphnia pulex, a widely distributed species that serves as an important model system in ecology, evolution, toxicology, and genomics
A simple salting out procedure for extracting DNA from human . . . A rapid and simple method for the isolation of high molecular weight cellular and chromosome-specific DNA in solution without the use of organic solvents Nucleic Acids Res 1987 Jan 26;15 (2):859–859 doi: 10 1093 nar 15 2 859
DNA PROTOCOLS FOR PLANTS Prof Jeffrey Doyle CT AB Total DNA . . . Separate from unincorporated dNTPs on Sephadex G100-120 column (track light blue Blue Dextran fraction, which migrates with high molecular weight DNA) [alternative methods, such as spun columns, are often used here]