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Agarose gel electrophoresis of RNA isolated using the . . . However, the length of the gels necessary to allow for good resolution of the low molecular weight RNA may be prohibitively large for use in some laboratories It has been demonstrated, and confirmed herein, that resolution of low molecular weight RNA molecules can be more effectively obtained on polyacrylamide gels (4)
Chapter 4 Preparation of RNA and Ribosomes from Yeast In the chapter, preparation of ribosomal subunits and polyribosomes and preparation of the high-molecular-weight rRNAs and low-molecular-weight rRNAs are discussed An overview of polyacrylamide gel electrophoresis of low-molecular-weight RNAs and high-molecular-weight rRNA precursors is also presented in the chapter
Chapter 14 Study Flashcards | Quizlet Study with Quizlet and memorize flashcards containing terms like Consider the diagram of the results from a DNA-RNA hybridization experiment for an unknown gene For this experiment, a mixture of DNA and mRNA has been heated to a point of denaturation and cooled such that the RNA can hybridize, or form a double helix, with DNA that has a complementary nucleotide sequence Using the image
Features of yeast RNA polymerase I with special consideration . . . Ribosomal RNAs (rRNAs) are structural components of ribosomes and represent the most abundant cellular RNA fraction In the yeast Saccharomyces cerevisiae, they account for more than 60 % of the RNA content in a growing cell The major amount of rRNA is synthesized by RNA polymerase I (Pol I) This enzyme transcribes exclusively the rRNA gene which is tandemly repeated in about 150 copies on
Transcriptional Interactions Between Yeast tRNA Genes . . . For quantification of low molecular weight RNAs, U6mg, U14, and sup2 + b total RNA (10 μg) was boiled in sample buffer (85% deionized formamide, 9 mM EDTA, and 0 1% xylene cyanol) and iced before loading onto 10% polyacrylamide, 8 M urea denaturing gels in 1X TTE (90 mM T ris base, 29 mM T aurine and 0 5 mM E DTA) running buffer
(PDF) Features of yeast RNA polymerase I with special . . . Ribosomal RNAs (rRNAs) are structural components of ribosomes and represent the most abundant cellular RNA fraction In the yeast Saccharomyces cerevisiae , they account for more than 60 % of the
Yeast Genetic Structures and Functions - Wiley Online Library In 1960, Monier, Stephenson, and Zamecnik devised an approach to isolate low‐molecular‐weight RNA from yeast by simple phenol extraction and precipitation of the soluble RNA from the aqueous phase with ethanol, which made this organism a most useful source for further work