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Protocol for Bicinchoninic Acid (BCA) Protein Assay The Bicinchoninic Acid (BCA) protein assay is a reliable and sensitive method for the quantification of total protein in a sample It is more sensitive than the Bradford assay and can detect protein concentrations as low as 0 5 μg mL
Protein Determination by the Bicinchoninic Acid (BCA) Method SCOPE This protocol applies to all regulated products requiring protein analysis by the BCA method DISCUSSION Proteins reduce alkaline Cu (II) to Cu (I) in a concentration dependent manner Bicinchoninic Acid is a highly specific chromagenic reagent for Cu (I) forming a complex with an absorbance maximum at 562 nm
BCA Assay and Lowry Assays - Thermo Fisher Scientific - US This BCA assay detects protein concentrations from 20–2,000 μg mL using a two-component system: Reagent A, a carbonate buffer containing BCA reagent, and Reagent B, a cupric sulfate solution, which are combined to make an apple green–colored working solution that turns purple after 30 minutes at 37°C in the presence of protein
BCA Assay BCA Principle: The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein
Protein Quantification: BCA Assay – QB3 Berkeley The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample In this assay, copper (II) [Cu2+] is reduced to copper (I) [Cu1+] in the presence of peptides in alkaline solutions
Bicinchoninic Acid (BCA) Protein Assay - G-Biosciences tergent solubilized protein solutions The Bicinchoninic Acid (BCA) Protein A say primarily relies on two reactions Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent rea
BCA Protein Quantification Protocol BCA Protein Quantification Protocol Prepare the BCA working reagent by mixing 50 parts of BCA Reagent A to 1 part of BCA Reagent B Example: 10mL of Reagent A with 200uL of Reagent B
User Guide: Pierce BCA Protein Assay Kit (MAN0011430 Rev. A) The Thermo ScientificTM PierceTM BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein
Pierce BCA Protein Assay Protocol This is the protocol to be used in the determination of total protein in tissue by the Pierce BCA method BSA (Bovine Serum Albumin) is used as the standard and samples are prepared by sonification in 1% homogenization buffer, the recipe for which is provided in the protocol below 1 1% Homogenization Buffer (HB)