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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR . . . C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage In vitro biochemical analysis show that C2c2 is guided by a single crRNA and can be programmed to cleave ssRNA targets carrying complementary protospacers
New CRISPR system for targeting RNA - MIT News The findings reveal that C2c2 — which is the first naturally occurring CRISPR system known to target only RNA, and was discovered by this collaborative group in October 2015 — helps protect bacteria against viral infection
Chinese English Pinyin Dictionary 吧一天啊 definition at Chinese Yabla com, a free online dictionary with English, Mandarin Chinese, Pinyin, Strokes Audio Look it up now!
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR . . . The mammalian codon-optimized gene for C2c2 (Leptotrichia shahii) was synthesized (GenScript, Jiangsu, China) and inserted into a bacterial expression vector (6-His-MBP-TEV, a pET based vector generously provided by Doug Daniels) using Golden Gate cloning
Two Distant Catalytic Sites Are Responsible for C2c2 RNase . . . - PubMed Here, we report the structures of Leptotrichia shahii C2c2 in its crRNA-free and crRNA-bound states While C2c2 has a bilobed structure reminiscent of all other Class 2 effectors, it also exhibits different structural characteristics
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR . . . C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage In vitro biochemical analysis show that C2c2 is guided by a single crRNA and can be programmed to cleave ssRNA targets carrying complementary protospacers