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Thermal shift assay - Wikipedia The assay is performed by incubating the protein with a radiolabelled ligand of the protein for 30 minutes at a given temperature, then quench on ice, run through a gel filtration mini column, and quantify the radiation levels of the protein that comes off the column
Practical: Investigating Temperature Enzyme Activity Revision notes on Practical: Investigating Temperature Enzyme Activity for the Edexcel IGCSE Biology syllabus, written by the Biology experts at Save My Exams
Thermal Shift Assay using SYPRO Orange to Detect Protein Melting . . . In addition, the thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO Orange This fluorescence dye is a naturally quenched dye and interacts with the hydrophobic core of proteins which is exposed after denaturation
Protein Thermal Shift technology - Thermo Fisher Scientific An assay was used to demonstrate the thermal stability of RecA in the presence of ATP used as a ligand (Figure 4) Results showed that the thermal stability of RecA increased from 53 2°C to 57 7°C in the presence of 5 mM ATP based on median derivative Tm values
AppNote-qTOWER3-thermal-shift-assays-en - Analytik Jena In contrast to a traditional CD Spectroscopy assay which takes approximately one hour per sample, the current experiment was performed in ten minutes for nine samples with excellent resolution of the investigated melting point
Thermal Shift Assay Services | Reaction Biology Upon binding compounds cause a considerable change in the melting temperature of the target protein These assays are carried out by our well-versed scientists with completion of standard studies within two weeks
Thermal Shift Assays (TSA) – NanoTemper Technologies Typically, the inflection points (IP) or melting points (Tm) of the protein are shifted towards higher temperatures upon interaction with a ligand, giving the assay its name