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  • Is the full crossmatch valid after saline replacement
    Hi all, We have a patient with strong rouleaux and an alloantibody According to our policy, we need to perform a full crossmatch which means the crossmatch is carried from immediate spin, to 37C and to AHG phases Due to the strong rouleaux, a saline replacement technique has to be used during t
  • can you tell if Rouleaux is present just from looking at the gel card . . .
    I apologize, this may be a stupid question For example, if you are trying to do a gel cross match and the donor cells have Rouleaux, can you tell that from looking at the gel card? If so, can you tell me where I can find some pictures of this reaction Thank you for your time and help in advance
  • Rouleaux or cold auto - Transfusion Services - PathLabTalk
    We perform our antibody screens at 37C and IAT only (since AABB STD's says methods that detect SIGNIFICANT antibodies) The problem is, that crossmatches are performed at Immediate Spin Cold autos are missed at antibody screening, and the screen is reported as negative When techs crossmatch units,
  • Rouleaux interference - Transfusion Services - PathLabTalk
    It's not uncommon to see rouleaux interference in Gel testing but it's not something we see frequently The crossmatch question is interesting: Technically, the screen is negative for alloantibody reactivity so I think you'd be able to follow your procedure for that result However, with the immediate spin crossmatch, you may have to perform a saline replacement to obtain a "compatible" unit
  • Rouleaux detected in IS crossmatch - PathLabTalk
    Our current crossmatch procedure requires that if we have reactivity at IS that is resolved with saline replacement (rouleaux) that we need to set up the tubes again and take testing through AHG phase If the patient has no history of clinically significant antibody and current antibody screen is
  • Rouleaux - Transfusion Services - PathLabTalk
    Is this a good enough, to prove that Rouleaux is causing the reactions in Gel? What would you recommend to indicate reactions are Rouleaux and not something else?
  • anti-A1 or rouleaux? - Transfusion Services - PathLabTalk
    Almost certainly because it is anti-A1, rather than rouleaux, or any other "cold" antibody specificity, such as anti-ALeb, but, come what may, unless the reactions are positive at STRICTLY 37oC, it wi
  • Rouleaux in Ortho Gel Testing - PathLabTalk
    We are seeing rouleaux fairly frequently that appears in one screen cell and not the other, and in some of the gel panel cells the same way This is in a Ortho IgG gel card ran on a Vision analyzer The Rouleaux is verified in tube under the scope We normally run a PEG tube screen and panel on t




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