|
- Importance of the Ca2+-binding sites in the N-catalytic domain of a . . .
The synchrotron radiation experiments were performed at the beam line BL38B1 in the SPring-8 with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (Proposal No 2007A1336, 2007B1117)
- Characterization of the amino acids essential for the photo- and . . .
On the basis of this knowledge, the optimization of the peptide sequence can be approached with respect to the application of such peptides for use in photo- radiation protection
- The initial step of the thermal unfolding of 3-isopropylmalate . . .
In the present T-jump Laue method, a protein crystal was heated extremely quickly by YAG laser radiation and Laue images were recorded within 40 ms The T-jump generated by the laser was estimated to be ~70°C, which is sufficient to initiate thermal unfolding of some proteins
- Improved thermostability of proteinase K and recognizing the . . .
Abstract Proteinase K (PRK) is a proteolytic enzyme that has been widely used in industrial applications However, poor stability has severely limited the
- Classification of G-protein coupled receptors at four levels
Abstract G-protein coupled receptors (GPCRs) are transmembrane proteins which via G-proteins initiate some of the important signaling pathways in a cell a
- Different elements of mini-helix 1 are required for human growth . . .
Incident radiation was at 285 nm Both studies were performed at 20°C in 10 mM Tris pH 8 2, 150 mM NaCl Data were normalized by calculating the ratio of the signal at each wavelength divided by the signal at 340 nm All spectra are averaged from three scans Fig 4
- Expression, characterization and structure determination of an active . . .
The X-ray source was a Rigaku RU-200 rotating-anode generator operating at 50 kV 90 mA, which provided monochromator filtered Cu Kα radiation of 1 54 Å wavelength
- Structural consequences of replacement of an α-helical Pro residue in
The data were collected at room temperature, using Cu Kα radiation The crystallographic statistics are given in Table I Structure solution, model building and refinement Structure solution was obtained after starting from the coordinates of wild-type thioredoxin (Katti et al , 1990) by molecular replacement using the program AmoRe (Navaza
|
|
|