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- CUT RUN sequencing - Wikipedia
CUT RUN sequencing combines antibody-targeted controlled cleavage by micrococcal nuclease with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins It can be used to map global DNA binding sites precisely for any protein of interest
- CUT RUN Overview - Cell Signaling Technology
CUT RUN is an in vivo method that uses a target-specific primary antibody and a Protein A-Protein G-Micrococcal Nuclease (pAG-MNase) to isolate specific protein-DNA complexes 1,2,3 It only takes 1 to 2 days to get from cell to DNA and can be automated for maximal throughput and reproducibility 4
- Complete Step-by-Step Guide to CUT RUN - EpiCypher
What is CUT RUN? CUT RUN, or Cleavage Under Targets and Release Using Nuclease, is a groundbreaking approach for ultra-sensitive genomic mapping of chromatin targets CUT RUN was developed in Dr Steven Henikoff’s group1-3, and builds off immunotethering technology (chromatin immunocleavage, or ChIC) developed by Dr Ulrich Laemmli4
- CUT RUN Complete Guide - Active Motif
CUT RUN (Cleavage Under Targets Release Using Nuclease) is an epigenetic method used to investigate the genome-wide distribution of various chromatin-associated proteins and their modifications CUT RUN is a derivative of chromatin immunocleavage (ChIC)
- cutandrun: Introduction - nf-core
nf-core cutandrun is a best-practice bioinformatic analysis pipeline for CUT RUN, CUT Tag, and TIPseq experimental protocols that were developed to study protein-DNA interactions and epigenomic profiling
- ChIC CUT amp;RUN-seq protocol | Abcam
Chromatin Immuno-Cleavage Cleavage Under Targets and Release Using Nuclease (ChIC CUT RUN) is a chromatin profiling technique used to analyze DNA-protein interactions While ChIP-seq uses sonication of fixed cells to fragment chromatin, ChIC CUT RUN-seq uses an enzyme (pAG-MNase)
- CUT RUN: An Improved Method for Studying Protein-DNA Interactions - Addgene
Enter CUT RUN, an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the limitations of ChIP-seq CUT RUN, which stands for ‘Cleavage Under Targets and Release Using Nuclease,’ was recently developed by the Henikoff lab at the University of Washington
- A modified CUT RUN protocol and analysis pipeline to identify . . . - Cell
CUT RUN is a recently developed in situ chromatin profiling technique that enables high-resolution chromatin mapping and probing Herein, we describe our adapted CUT RUN protocol for transcription factors (TFs)
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