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- Phosphate buffer calculation help - Physics Forums
This isn't homework It's for my job I'm supposed to be making a 50mM phosphate buffer to pH 7 2, 0 5 NaOH I have access to salts, K2HPO4 (dibasic), KH2PO4 (monobasic), and NaCl I've determined that I need two liters of buffer solution I did the calculations but when I measured the pH after
- How Does Temperature Affect pKa Values in Buffer Preparations?
You can try another buffer calculator, which does make corrections for both temperature and ionic strength Note, that these theoretical values are almost always a bit off, theories we use are quite good for diluted solutions, but for concentrations higher that 0 1 M they start to fail
- Potassium Phosphate Buffer - Physics Forums
So I want to make a Potassium Phosphate Buffer from monobasic and dibasic forms of potassium phosphate because it was recommended for doing circular dichroism scans due to its low absorption in the UV region However I don't understand the proportions of mono basic and dibasic forms of potassium
- Henderson-Hasselbalch phosphate buffers - Physics Forums
This is an example calculation about the phosphate buffer system from my Biochemistry textbook Homework Statement If the total cellular concentration of phosphate is 20 mM (millimolar) and the pH is 7 4, the distribution of the major phosphate species is given by pH = pKa + log10
- Phosphate Buffer Concentration Calculation - Physics Forums
Borek said: Your approach is correct But: OK Check your math -- buffer calculator, concentration calculator pH calculator, stoichiometry calculator
- Preparing 0. 3M Phosphate Buffer at pH 7. 9: c), d), e), f)
According to theory, which amounts of the following acid and its conjugate base would you need to prepare 150 ml of a 0 3 M phosphate buffer at pH 7 9? Use the pKa table below to help you in your answer
- Calculate pH of Media Buffer Solution - Physics Forums
I think you can think you have your 2 3 mole of phosphate buffer at pH = pK = 7 21 and to it are adding 1 3 mole of H 2 PO 4- and 1 3 mole NH 4+ This added H 2 PO 4- stays in that form on the basis its dissociable proton 'has nowhere to go' The ammonia is already protonated and if it protonates a HPO 42- the result is no change!
- Citric acid buffer solution for prac - Physics Forums
Hi Guys, Citric acid has three different values for pKa because it is a polyprotic acid I am aiming to make a buffer solution with sodium citrate but I am not sure which pKa value to use in the Henderson-Hasselbalch equation Does anyone have any ideas?
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