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- Leucine-rich repeat kinase 2 (LRRK2) - ALZFORUM
LRRK2 is also expressed in circulating immune cells, pointing to a role in innate immunity Indeed, LRRK2 has also been linked to Crohn’s and other inflammatory bowel diseases Leucine-rich repeat kinase 2 encodes a large, complex protein containing a kinase, a GTPase, and multiple protein-protein interaction domains
- LRRK2 G2019S Mouse (BAC Tg) - ALZFORUM
This transgenic (Tg) mouse overexpresses a mutant form of Lrrk2 (G2019S) using a bacterial artificial chromosome (BAC) (Li et al , 2010) Transgene expression is driven by the mouse Lrrk2 promoter sequence Hemizygous mice develop an age-associated decrease in striatal dopamine, but no loss of
- LRRK2 G2019S Mouse (Tg) - ALZFORUM
LRRK2 protein was found at levels about 2 7-fold higher than endogenous LRRK2 levels in tyrosine hydroxylase (TH)-positive dopaminergic neurons of the substantia nigra pars compacta In whole brain samples from 2-month-old transgenic mice, LRRK2 is expressed at levels of 3- to 4-fold higher than non-transgenic animals (Nikonova et al , 2012)
- LRRK2 R1441C Mouse (Tg - Conditional) - ALZFORUM
R26-LRRK2 + + mice harbor a transgene containing a full-length mutant human LRRK2 preceded by a floxed stop cassette, inserted into the ROSA26 locus; these mice allow for conditional expression of human LRRK2 R1441C when crossed with mice carrying a transgene for Cre recombinase These transgenic
- LRRK2 WT Mouse (BAC Tg) - ALZFORUM
The LRRK2 mouse uses a BAC system to express WT or R1441C LRRK2, which is a mutation in the GTPase domain of LRRK2 The benefit of the BAC system is that it permits use of the LRRK2 promoter, which allows for an expression pattern that is elevated but exhibits a distribution that recapitulates the pattern of endogenous LRRK2
- LRRK2 G2019S Rat (BAC Tg) - ALZFORUM
This transgenic (Tg) rat model overexpresses mutant Lrrk2 It was originally developed in the laboratory of Chenjian Li and then re-derived at Taconic with the support of the Michael J Fox Foundation This model expresses high levels of mutant Lrrk2 in the brain An early study found approximately
- Lrrk2 KO Mouse - ALZFORUM
Lrrk2 KO mice were generated by Cre-mediated deletion of coding exon 2 of the Lrrk2 gene, which results in a premature stop codon in exon 3 (Lin et al , 2009) Heterozygous KO mice were intercrossed to generate homozygous KO mice (Parisiadou et al , 2009) This knock-out (KO) model was generated by
- Sigh of Relief? Lung Effects of LRRK2 Inhibitors are Mild.
Proponents of LRRK2 inhibitor programs may breathe easier following a paper in the April 22 Science Translational Medicine A collaboration led by researchers at the Michael J Fox Foundation found that three different inhibitors of this Parkinson’s-linked kinase caused morphological changes in
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