- Tissue-Specific Targeting of the Pthrp Gene: The Generation . . .
By combining thecre loxP site-specific recombination system with embryonic stem (ES) cell technology, the capability of achieving conditional gene targeting has greatly expanded
- Genotyping of Cre-lox Mice and Detection of Tissue-Specific . . .
Conditional gene targeting, based on Cre-lox or other systems, requires frequent genotyping of transgenic mouse popula-tions and monitoring of tissue-specific Cre recombinatory efficiency This is currently achieved by Southern analysis from tail-and tissue-derived DNA
- Generation and Validation of Tissue-Specific Knockout Strains . . .
Here, we give a detailed description of the steps required to generate and validate tissue-specific knockout mice using the Cre-loxP system Basic Protocol 1 describes how to use gene targeting in mouse embryonic stem cells in order to generate mice with conditional alleles
- CLICK: one-step generation of conditional knockout mice
Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats
- Alternative splicing of parathyroid hormone-related protein . . .
The production of the three different PTHrP isoforms by alternative splicing is one mechanism to achieve cell type and tissue-specific regulation, which may be altered during carcinogenesis or cancer progres-sion
- The midregion, nuclear localization sequence, and C terminus . . .
Mice with ablation of the Pthrp gene (Pthrp ) are characterized by chondrodysplasia, ac-celerated chondrocyte differentiation and endochon-dral ossification, and lethality within minutes of birth, demonstrating that PTHrP is critical for survival and skeletal morphogenesis (7, 8)
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