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- Research Techniques Made Simple: Polymerase Chain Reaction (PCR)
Introduction The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990) For the first time, it allowed for specific detection and production of large amounts of DNA PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project
- DNA Extraction and Polymerase Chain Reaction - PMC
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in
- Polymerase Chain Reaction (PCR) - PubMed
The polymerase chain reaction (PCR) is a frequently utilized laboratory nucleic acid amplification technique that uses Taq polymerase, a thermostable DNA polymerase isolated from <i>Thermus aquaticus< i>, to synthesize DNA following thermal denaturation and primer annealing Kary Mullis introduced P …
- PCR - the polymerase chain reaction - PubMed
DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique Developed in 1985 by Kary B Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied Mullis's invention …
- PCR in Forensic Science: A Critical Review - PMC
The polymerase chain reaction (PCR) has played a fundamental role in our understanding of the world, and has applications across a broad range of disciplines The introduction of PCR into forensic science marked the beginning of a new era of DNA
- Principles and applications of polymerase chain reaction in . . .
Abstract Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements PCR is an excellent technique for
- The polymerase chain reaction - PubMed
The polymerase chain reaction (PCR) is a novel technique that amplifies specific sequences with remarkable efficiency Repeated cycles of denaturation, primer annealling and extension carried out with the heat stable enzyme, Taq polymerase, leads to exponential increases in the target DNA sequences
- POLYMERASE CHAIN REACTION - PMC
Polymerase chain reaction (PCR) is a technology for exponential amplification of a fragment of DNA (The PCR is covered by patents owned by Hoffman-La Roche A license is required to use the PCR process ) The limit of its sensitivity is a single molecule, making PCR a superb qualitative tool for the specific detection of rare DNA sequences
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