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- pGEX-parkin K161N - Addgene
Plasmid pGEX-parkin K161N from Dr Kalle Gehring's lab contains the insert Parkin K161N and is published in Commun Biol 2024 Aug 8;7 (1):961 doi: 10 1038 s42003-024-06656-x This plasmid is available through Addgene
- BECN1 is involved in the initiation of mitophagy: It facilitates PARK2 . . .
Measurements of the level of MFN2 (mitofusin 2), a PARK2 substrate, demonstrate that depletion of BECN1 prevents PARK2 translocation-induced MFN2 ubiquitination and loss BECN1 depletion also rescues the MFN2 loss-induced suppression of mitochondrial fusion
- NM_004562. 3 (PRKN):c. 483A gt;T (p. Lys161Asn) AND Autosomal recessive . . .
Of 8 novel mutations identified in the PARK2 gene in European families with autosomal recessive juvenile parkinsonism (600116) by Abbas et al (1999), 1 of the 4 missense mutations was lys161 to asn (K161N)
- PINK1 Parkin-mediated mitophagy is dependent on VDAC1 and p62 . . . - Nature
Consistently, the execution-deficient mutants K161N and R275W were recruited to mitochondria similarly to wild-type Parkin, whereas the translocation-compromised mutant G430D failed
- (PDF) The TOMM machinery is a molecular switch in PINK1 and PARK2 . . .
Consistent with FRET measurements, the presence of fluorescently labeled PARK2 or its K161N variant, but not the K211N and R256C variants, significantly decreased the mean lifetime of TOMM70A fluorescence; normal PARK2 had a stronger effect than the K161N variant
- Addgene: pLv-EF1a-Parkin (K161N)-A92mKO2
Plasmid pLv-EF1a-Parkin (K161N)-A92mKO2 from Dr Lani Wu's lab contains the insert Parkin and is published in Cell Rep 2023 Oct 31;42 (10):113260 doi: 10 1016 j celrep 2023 113260 Epub 2023 Oct 17 This plasmid is available through Addgene
- Parkin represses 6-hydroxydopamine-induced apoptosis via stabilizing . . .
Our results showed that EGFP-parkin, but not EGFP-parkin K161N or EGFP-parkin T240R, increased p62 levels (Figure 2B) These results suggest that parkin stabilizes p62 through its E3 ligase
- Comparison of ∼110-kDa complex formation between wild . . . - ResearchGate
Comparison of ∼110-kDa complex formation between wild-type (WT) parkin and PD-linked parkin variants COS1 cells were transiently transfected with 70 ng cm2 of WT or R256C mutant parkin cDNA, 50
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