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- 抗体的Cy5 荧光素标记教程 - 知乎
本试剂盒主要通过 荧光素基团 的活性键与生物分子的游离氨基共价结合，可以用于标记抗体、 蛋白等其它含游离氨基生物分子。 操作过程 ·实验前准备 1 仔细阅读使用说明书。 2 试剂耗材准备：提前 20 min 从冰箱中取出试剂盒，平衡至室温（注：不需要用到的试剂组分继续放置在冰箱中）。 3 超滤管 浸润：向干燥的超滤管滤芯中加入标记缓冲液，室温放置10 min备用，在加入待标记物之前弃去标记缓冲液即可（整个标记过程中超滤管滤芯都应该保持湿润）。 4 溶解活化 Cyanine 5 干粉（以产品规格为0 5 mg为例）：用40 μL DMSO溶解Cyanine 5干粉，静置 10 min，待其充分溶解，此时Cyanine 5的浓度为0 77 mM，盖好管子备用。
- Fluorescent labeling of plasmid DNA for gene delivery: Implications of . . .
We developed a novel fluorescent labeling strategy utilizing an aryl azide photolabel conjugated to a short cationic peptide to label plasmid DNA with Cyanine 5 and sulfo-Cyanine 5 Using a simple camera flash apparatus, photolabel-peptide-dyes can be conjugated to DNA in minutes with preservation of DNA structure and minimal dye photobleaching
- ReadiLink Cy5 Oligo 和 ssDNA 标记试剂盒 | 西安百萤生物 . . .
ReadiLink Cy5 Oligo 和 ssDNA 标记试剂盒能够使用 Cy5 荧光团对单链 DNA 或寡核苷酸进行简单而统一的标记。 标记试剂盒使用我们专有的 TAQuest 末端脱氧核苷酸转移酶 (TdT) 催化非模板定向核苷酸掺入到单链 DNA 或寡核苷酸的 3'-末端。
- Elabscience Cyanine 5 (CY5)标记试剂盒 Cyanine 5 Labeling Kit
Elabscience® Cyanine 5 (CY5)标记试剂盒提供了标记所需的全部试剂,能简单有效地对含有伯氨基(-NH2)分子的蛋白标记。 快速:整个过程仅需90 min。 方便:染料已活化,可直接使用;Filtration tube脱盐,无需透析。 使用灵活:既可用于微量标记又可大量标记,每次可标记0 1-1 mg 蛋白。 水溶性:本试剂盒内Cyanine 5 为水溶性,在含水缓冲液中,染料荧光特性能较好测量及保持。 在一定pH 范围内,Cyanine 5 专一地与伯氨基反应(N-末端及赖氨酸残基侧链)形成稳定的酰胺键, 从而实现与蛋白的偶联。 *Filtration tube 购自Millipore, 使用方法参考附录三。
- Label IT® Tracker Intracellular Nucleic Acid Localization Kit, Cy5 . . .
Tracking of Plasmid Localization and Expression COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and Trans IT®-LT1 Reagent in serum containing media Images were acquired at 3, 8, and 20 hours post-transfection
- Cy5 NT Labeling Kit, Fluorescent Nick Translation Labeling Kits - Jena . . .
The Cy5 NT labeling mix contains specially optimized dUTP-Cy5 for incorporation into DNA by nick translation using DNA Polymerase I The excellent stability and quantum yield of the fluorophore combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications
- Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of . . .
We developed a novel fluorescent labeling strategy utilizing an aryl azide photolabel conjugated to a short cationic peptide to label plasmid DNA with Cyanine 5 and sulfo-Cyanine 5 Using a simple camera flash apparatus, photolabel-peptide-dyes can be conjugated to DNA in minutes with preservation of DNA structure and minimal dye photobleaching
- Highly chromophoric Cy5-methionine for N-terminal fluorescent . . . - Nature
Cy5-Met-initiator tRNA (Cy5-Met-tRNAi) was purified and transfected into HeLa cells with HIV-Tat plasmid, resulting in an efficient production of Cy5-labeled HIV-Tat protein
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