|
- Overview of ELISA | Thermo Fisher Scientific - US
ELISPOT (enzyme-linked immunospot assay) refers to ELISA-like capture and measurement of proteins secreted by cells that are plated in PVDF-membrane-backed microplate wells It is a "sandwich" assay in which the proteins are captured locally as they are secreted by the plated cells, and detection is with a precipitating substrate
- ELISA technical guide and protocols - Thermo Fisher Scientific
The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies
- ELISA Guides and Resources | Cell Signaling Technology
Enzyme-linked immunosorbent assay (ELISA) allows for the quantification of a molecule of interest using antibodies and tags (such as the enzyme horseradish peroxidase [HRP]), either directly or indirectly coupled to the antibody for detection ELISA can be used with a variety of sample types, such as cell lysate, tissue lysate, or serum, and
- In-cell ELISA protocol - Abcam
Discover our In-Cell ELISA protocol, used to measure protein levels or post-translational modifications of cultured adherent cells Adherent cells can be grown and treated directly in the assay microplate Cell attachment can be checked with a microscope Correct the raw in-cell ELISA signal for the background by subtracting the mean
- ELISA Assays: Methods, Analysis, Lab Techniques, and Applications
Cell-based ELISA assays are convenient, sensitive, high-throughput, and lysate-free They can measure relative cellular protein phosphorylation Besides, cell-based ELISA assays can monitor the impact of inhibitors, activators, or therapeutic interventions on phosphorylation Cell-based assays can evaluate several cellular biochemical processes
- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA . . . - PubMed
The cell-based enzyme-linked immunosorbent assay (cell-ELISA) combines steps of direct immunochemical labelling of cell cultures and ELISA It can be used for quantitative comparisons of surface-expressed and total protein contents of various cell cultures While several cell-ELISA protocols are available for different cell types, in this
- Cell-Based ELISA Assay - Creative Bioarray
The intensity of the resulting signal is directly proportional to the concentration of phosphorylated protein present in the original sample Cell-based ELISA is a convenient, lysate-free, high throughput and sensitive assay that can measure the relative amount of protein phosphorylation in cells
- Automation of a Novel Cell-Based ELISA for Cell Signaling . . . - Agilent
Protein kinases involved in these cellular cascades play many diverse biological roles including normal growth and development; their aberrant behavior is linked to a number of infirm states including cancer A novel one wash step ELISA assay has been developed for detection of cell-based signal transduction events In addition to offering
|
|
|