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- The Purpose of ROX Reference Dye in Real-Time PCR (qPCR)
ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product
- 荧光定量PCR—荧光篇 - 知乎
ROX(Passive Reference Dye)是一种惰性参比染料,不参与荧光定量PCR反应,荧光信号值不会随反应而改变。 由于耗材质量、机器稳定性、照明不均匀、反应体系的浓度等原因造成每个样品孔会有微小的差异,ROX可作为阳性参比,对荧光信号进行归一化校正。
- 荧光定量PCR研究——ROX染料是什么作用? - 分析测试百科网
ROX(Passive Reference Dye)是一种惰性参比染料,在qPCR反应中能被qPCR仪检测到。 ROX不参与qPCR反应,荧光信号值不会随着qPCR扩增而改变。
- 什么是Rox?qPCR Mix是否含有ROX,你选对了么?
ROX(Passive Reference Dye)是一种惰性参比染料,在PCR反应中能被PCR仪检测到。 它不参与PCR反应,且荧光信号值是稳定的,不会随着PCR扩增发生改变。
- What Is ROX And Why Use It In qPCR? – SignaGen Blog
ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix Unlike reporter dyes in qPCR (e g SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions
- Normalization of Real-Time PCR Fluorescence Data with ROX Passive . . .
ROX (carboxy-X-rhodamine) is used as a passive reference dye in ROX-dependent real-time PCR systems to normalize for differences in fluorescence levels that can occur due mainly to optical path variations among wells
- ROX passive reference dye for troubleshooting real-time PCR
ROX passive reference dye for troubleshooting real-time PCR PCR)—is one of the most powerful and sensitive gene analysis techniques available Real-time quantitation of DNA and cDNA requires monitoring of a fluores ent signal that reports on the progress of the amplification reaction at each cycle (For
- 分子 | ROX染料在RT-PCR中的作用 - 知乎
大家好,今天我们来讨论一下,我们经常提到的ROX是什么东西,以及它在 RT-PCR 中的功能是什么? 和 FAM, VIC 这样的数字荧光染料很像,ROX也是一种 荧光分子,当它出现在反应中时,实时PCR仪器可以检测到它发出的信号值。
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