|
- The Purpose of ROX Reference Dye in Real-Time PCR (qPCR)
ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product
- 荧光定量PCR—荧光篇 - 知乎
ROX（Passive Reference Dye）是一种惰性参比染料，不参与荧光定量PCR反应，荧光信号值不会随反应而改变。 由于耗材质量、机器稳定性、照明不均匀、反应体系的浓度等原因造成每个样品孔会有微小的差异，ROX可作为阳性参比，对荧光信号进行归一化校正。
- 荧光定量PCR研究——ROX染料是什么作用？ - 分析测试百科网
ROX（Passive Reference Dye）是一种惰性参比染料，在qPCR反应中能被qPCR仪检测到。 ROX不参与qPCR反应，荧光信号值不会随着qPCR扩增而改变。
- 什么是Rox？qPCR Mix是否含有ROX，你选对了么？
ROX（Passive Reference Dye）是一种惰性参比染料，在PCR反应中能被PCR仪检测到。 它不参与PCR反应，且荧光信号值是稳定的，不会随着PCR扩增发生改变。
- What Is ROX And Why Use It In qPCR? – SignaGen Blog
ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix Unlike reporter dyes in qPCR (e g SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions
- Normalization of Real-Time PCR Fluorescence Data with ROX Passive . . .
ROX (carboxy-X-rhodamine) is used as a passive reference dye in ROX-dependent real-time PCR systems to normalize for differences in fluorescence levels that can occur due mainly to optical path variations among wells
- ROX passive reference dye for troubleshooting real-time PCR
ROX passive reference dye for troubleshooting real-time PCR PCR)—is one of the most powerful and sensitive gene analysis techniques available Real-time quantitation of DNA and cDNA requires monitoring of a fluores ent signal that reports on the progress of the amplification reaction at each cycle (For
- 分子 | ROX染料在RT-PCR中的作用 - 知乎
大家好，今天我们来讨论一下，我们经常提到的ROX是什么东西，以及它在 RT-PCR 中的功能是什么？ 和 FAM， VIC 这样的数字荧光染料很像，ROX也是一种 荧光分子，当它出现在反应中时，实时PCR仪器可以检测到它发出的信号值。
|
|
|